Determining the origin of macadamia kernels from market samples (MC01002)
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What was it all about?
There were two parts to this project; one was to establish a quick and efficient method of extracting DNA from processed kernel; the other was to demonstrate that the extracted DNA could be genotyped using a robust fingerprinting method.
Using a commercial DNA extraction protocol (DNeasy Extraction Protocol, QIAGEN Pty Ltd) the researcher generated high quality DNA from raw and air-roasted kernel that could be readily and easily genotyped at microsatellite loci. Oil-roasted kernel samples generated significantly lower quantities of DNA, but it was still possible to genotype these samples at microsatellite loci.
Use of co-dominant microsatellite loci provided a range of advantages over previously employed dominant markers (Jarne & Lagoda 1996; Parker et al. 1998) and, when genotypes of processed kernel were compared to reference genotypes drawn from different growing regions, it allowed for the identification of kernel source/origin.
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This project was funded by Hort Innovation (then Horticulture Australia Limited) with the voluntary financial support of the Macadamia Industry.
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