Determining the origin of macadamia kernels from market samples (MC01002)
This is a final research report from Hort Innovation’s historical archives. Please note that as these reports may date back as far as the 1990s, the content and recommendations within them may be superseded by more recent research.
What was it all about?
There were two parts to this project; one was to establish a quick and efficient method of extracting DNA from processed kernel; the other was to demonstrate that the extracted DNA could be genotyped using a robust fingerprinting method.
Using a commercial DNA extraction protocol (DNeasy Extraction Protocol, QIAGEN Pty Ltd) the researcher generated high quality DNA from raw and air-roasted kernel that could be readily and easily genotyped at microsatellite loci. Oil-roasted kernel samples generated significantly lower quantities of DNA, but it was still possible to genotype these samples at microsatellite loci.
Use of co-dominant microsatellite loci provided a range of advantages over previously employed dominant markers (Jarne & Lagoda 1996; Parker et al. 1998) and, when genotypes of processed kernel were compared to reference genotypes drawn from different growing regions, it allowed for the identification of kernel source/origin.
ISBN:
0 7341 1201 7
Funding statement:
This project was funded by Hort Innovation (then Horticulture Australia Limited) with the voluntary financial support of the Macadamia Industry.
Copyright:
Copyright © Horticulture Innovation Australia Limited 2005. The Final Research Report (in part or as whole) cannot be reproduced, published, communicated or adapted without the prior written consent of Hort Innovation (except as may be permitted under the Copyright Act 1968 (Cth)).