Improving diagnostics and biosecurity for graft-transmissible diseases in citrus (CT17007)
What’s it all about?
This project is responsible for supporting the NSW DPI Citrus Pathology Program in strengthening biosecurity against graft-transmissible diseases, in collaboration with other bodies including Auscitrus. The team’s activities include:
- Working towards improved detection and diagnostics of graft-transmissible pathogens of citrus, including through assessing, developing and validating lab diagnostic procedures
- Understanding the risk posed by newly discovered or reported graft-transmissible diseases
- Responding to industry requests for diagnostic support.
The work follows on from earlier Hort Innovation Citrus Fund investment Protecting Australian citrus germplasm through improved diagnostic tools project (CT14009) and ties into other investments including Improving biosecurity preparedness of the Australian citrus industry (CT17001).
Commencing in early 2019, activities are well underway in strengthening biosecurity against graft-transmissible diseases in citrus. To date, the project team have:
- Reviewed and updated the list of Australian citrus pathogens held in the NSW DPI collection to include additional pathogens not previously registered
- Conducted a successful trial to evaluate a new rapid method of testing citrus viroids and viruses, finding that it is considerably cheaper to perform and would be useful in situations where resources are limited
- Worked on the development of a field-based test for detecting a wide range of citrus viroid strains, with greater knowledge of the diversity of Australian viroid and viral strains achieved with the help of international experts
- Established pot trials for ongoing research to determine the host range and potential impact of the newly discovered citrus viroid VII (CVd-VII)
- Collected budwood from wilting trees at an orchard in Dareton NSW, which was submitted for diagnostic testing for citrus virus A (CiVA) and citrus concave gum associated virus (CCGAsV)
- Sampled nursery trees at the Department of Agriculture and Fisheries Queensland’s Bundaberg Research Station and field trees from far North Queensland to send to South Africa for citrus tristeza virus (CTV) strain characterisation.
- Assisted an international collaborator by validating a newly developed diagnostic tool for the early detection of huanglongbing (HLB), with further details to be provided when results are finalised
- Successfully detected citrus stubborn disease using a new method provided by the University of California, Riverside, with other tests to now be trialled
- Continued to process samples for the Plant Health Diagnostic Services and respond to industry enquiries.
This project is a strategic levy investment in the Hort Innovation Citrus Fund