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Completed project

Developing virus molecular diagnostics for post entry quarantine and certification of strawberry runners (BS12009)

Key research provider: The Victorian Department of Economic Development, Jobs, Transport and Resources
Publication date: Friday, May 26, 2017

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What was it all about?

This project, which ran from 2013 to 2016, focused on the biosecurity of the Australian strawberry industry, with the development of improved tools to underpin quarantine access to planting material, supporting the certification of strawberry runners. It was largely funded by voluntary contributions, with some levy funding.

It resulted in the production of a draft diagnostic manual, submitted for review and subsequent endorsement by the National Plant Biosecurity Diagnostic Network, to be integrated into the Victorian Strawberry Industry Certification Authority scheme.

The methods for pathogen detection include…

  • Visual observations
  • Biological indexing onto sensitive indicator plants
  • Culturing onto agar plates for fungal and bacterial pathogens
  • Molecular methods such as polymerase chain reaction (PCR) tests and reverse transcription (RT)-PCR tests.

The manual incorporates information of horticulture practices to support biological indexing of viruses and fungi, gives guidance on optimal sampling and testing procedures with respect to cool temperate and sub-tropical climates, and gives specific protocols for a range of pathogens.

Related levy funds
Details

ISBN:
978-0-7341-3948-1

Funding statement:
This project has been funded by Hort Innovation

Copyright:
Copyright © Horticulture Innovation Australia Limited 2017. The Final Research Report (in part or as whole) cannot be reproduced, published, communicated or adapted without the prior written consent of Hort Innovation (except as may be permitted under the Copyright Act 1968 (Cth)).